ACTA MEDICA
ACTA MEDICA
Acta Medica (Hradec Králové) is an English language multidisciplinary medical journal. Acta Medica publishes reviews, original articles, brief communications, case reports, announcements, and notices. The journal was founded in 1958 under the title “A Collection of Scientific Works of the Charles University’s Faculty of Medicine in Hradec Králové”. The journal is indexed in Chemical Abstracts, CNKI, DOAJ, EBSCO, Hinari, Index Medicus, MEDLINE, Scopus, and Ulrichsweb.

ACTA MEDICA, Vol 59 No 4 (2016), 107–112

Histological Evaluation of Decellularized Skeletal Muscle Tissue Using Two Different Decellularization Agents

Hana Hrebíková, Magda Voborníková, Milada Hetešová, Jaroslav Mokrý

DOI: https://doi.org/10.14712/18059694.2017.36
published online: 22. 02. 2017

abstract

The aim of the present study was to determine effect of two decellularized agents, sodium dodecyl sulphate (SDS) and Triton X-100, to the skeletal muscle tissue. Final scaffold was evaluated by several histological techniques to analyse preservation of essential structures including collagen and elastic fibres, basement membranes, glycosaminoglycans and also to confirm elimination of nuclear and cytoplasmic components which are redundant in effectively prepared decellularized scaffolds. Comparison of tissue scaffolds processed with different detergents proved that SDS is superior to Triton X-100 as it can effectively decellularize muscle tissue.

keywords: Decellularized scaffold; Histology; Skeletal muscle; SDS; Triton X-100

Creative Commons License
Histological Evaluation of Decellularized Skeletal Muscle Tissue Using Two Different Decellularization Agents is licensed under a Creative Commons Attribution 4.0 International License.

210 x 297 mm
periodicity: 4 x per year
print price: 150 czk
ISSN: 1211-4286
E-ISSN: 1805-9694

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